作者： Hongzhao Qi†, Chaoyong Liu⊥, Lixia Long†, Yu Ren‡, Shanshan Zhang†, Xiaodan Chang†, Xiaomin Qian†, Huanhuan Jia‡, Jin Zhao†, Jinjin Sun§, Xin Hou†, Xubo Yuan†, and Chunsheng Kang⊥
‡ Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China
§ Department of Surgery, Second Hospital of Tianjin Medical University, Tianjin 300211, China
⊥ Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Department of Neurosurgery, Tianjin Medical University General Hospital and Key Laboratory of Neurotrauma, Variation, and Regeneration, Ministry of Education and Tianjin Municipal Government, Tianjin 300052, China
摘要：Exosomes are a class of naturally occurring nanoparticles that are secreted endogenously by mammalian cells. Clinical applications for exosomes remain a challenge because of their unsuitable donors, low scalability, and insufficient targeting ability. In this study, we developed a dual-functional exosome-based superparamagnetic nanoparticle cluster as a targeted drug delivery vehicle for cancer therapy. The resulting exosome-based drug delivery vehicle exhibits superparamagnetic behavior at room temperature, with a stronger response to an external magnetic field than individual superparamagnetic nanoparticles. These properties enable exosomes to be separated from the blood and to target diseased cells. In vivo studies using murine hepatoma 22 subcutaneous cancer cells showed that drug-loaded exosome-based vehicle delivery enhanced cancer targeting under an external magnetic field and suppressed tumor growth. Our developments overcome major barriers to the utility of exosomes for cancer application.
關鍵詞：cancer targeting; drug delivery; exosome; scalable separation; superparamagnetic nanoparticle clusters
